Loading...
|
Please use this identifier to cite or link to this item:
https://irlib.pccu.edu.tw/handle/987654321/2716
|
| Title: | Macrophages derived from bone marrow modulate differentiation of myeloid dendritic cells |
| Authors: | Liao, H. -F.
Yang, Y. -C.
Chen, Y. -Y.
Hsu, M. -L.
Shieh, H. -R.
Chen, Y. -J. |
| Contributors: | 運教所 |
| Keywords: | macrophage
dendritic cell
differentiation
interaction
bone marrow |
| Date: | 2007 |
| Issue Date: | 2009-11-16 11:07:43 (UTC+8) |
| Abstract: | Dendritic cells (DC) are specialized antigen-presenting cells. Bone marrow monocytes have been widely used to generate murine myeloid DC. We found that mouse macrophages derived from bone marrow CD11b(+) monocytes influenced the differentiation of these precursors into DC. Modulation of differentiation was demonstrated by the down-regulation of CD11c, CD40, and CD86 expression and by IL-12 production. DC differentiated in the presence of conditioned medium from bone marrow-derived macrophage culture (MCM) had impaired ability to stimulate proliferation of, and IFN-gamma production by, allogeneic CD4(+) T cells. This inhibition of DC differentiation was mainly mediated by secretory products from macrophages but not by cell-cell contact. MCM contained higher concentrations of macrophage-colony-stimulating factor (M-CSF), IL-10, and TGF-beta 1, whereas IL-6 remained unchanged compared with conditioned medium from fresh monocytes. M-CSF may be the major mediator in MCM inhibiting DC differentiation. This study demonstrates an important influence of bone marrow-derived macrophages on DC precursors during DC differentiation. |
| Relation: | CELLULAR AND MOLECULAR LIFE SCIENCES Volume: 64 Issue: 1 Pages: 104-111 |
| Appears in Collections: | [Graduate Institute of Sport Coaching Science ] journal articles
|
Files in This Item:
| File |
Description |
Size | Format | |
|---|
| index.html | | 0Kb | Text | 596 | View/Open |
|
All items in CCUR are protected by copyright, with all rights reserved.
|
