資料載入中.....
|
請使用永久網址來引用或連結此文件:
https://irlib.pccu.edu.tw/handle/987654321/2697
|
| 題名: | Growth inhibition and induction of apoptosis in NB4 promyelocytic leukemia cells by trypsin inhibitor from sweet potato storage roots |
| 作者: | Huang, Guan-Jhong
Sheu, Ming-Jyh
Chen, Hsien-Jung
Chang, Yuan-Shiun
Lin, Yaw-Huei |
| 貢獻者: | 園生系 |
| 關鍵詞: | sweet potato
trypsin inhibitor
apoptosis
NB4
caspase |
| 日期: | 2007 |
| 上傳時間: | 2009-11-13 15:34:06 (UTC+8) |
| 摘要: | The objective of this study was to investigate the antiproliferative effect and the mechanism of trypsin inhibitor (TI) from sweet potato [Ipomoea batatas (L.) Lam. 'Tainong 57'] storage roots on NB4 promyelocytic leukemia cells. The results showed that TI inhibited cellular growth of NB4 promyelocytic leukemia cells in a time-dependent and dose-dependent manner, and treatment for 72 h induced a marked inhibition of cellular growth, showing an IC50 of 57.1 +/- 8.26 mu g/mL. TI caused cell cycle arrest at the G1 phase as determined by flow cytometric analysis and apoptosis as shown by DNA laddering. TI-induced cell apoptosis involved p53, Bcl-2, Bax, and cytochrome c protein in NB4 cells. P53 and Bax proteins were accumulated, and antiapoptotic molecule Bcl-2 was decreased in the tested cells in a time-dependent manner during TI treatment. TI also induced a substantial release of cytochrome c from the mitochondria into the cytosol. Hence, TI induced apoptosis in NB4 cells through a mitochondria-dependent pathway, which was associated with the activation of caspase-3 and -8. These results demonstrated that TI induces NB4 cell apoptosis through the inhibition of cell growth and the activation of the pathway of caspase-3 and -8 cascades. |
| 關聯: | JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY Volume: 55 Issue: 7 Pages: 2548-2553 |
| 顯示於類別: | [園藝暨生物技術學系] 期刊論文
|
文件中的檔案:
| 檔案 |
描述 |
大小 | 格式 | 瀏覽次數 |
|---|
| index.html | | 0Kb | Text | 587 | 檢視/開啟 |
|
在CCUR中所有的資料項目都受到原著作權保護.
|
