Medium optimization of carbon and nitrogen sources for the production of spores from Bacillus amyloliquefaciens B128 using response surface methodology

文化大學機構典藏 CCUR > 環境規劃暨生物資源學院 > 農學院 > 園藝暨生物技術學系 > 期刊論文 > Item 987654321/2694

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题名:	Medium optimization of carbon and nitrogen sources for the production of spores from Bacillus amyloliquefaciens B128 using response surface methodology
作者:	Rao, Yerra Koteswara Tsay, Kuen-Juh Wu, Wen-Shi Tzeng, Yew-Min
贡献者:	園生系
关键词:	bacillus amyloliquefaciens B128 spore response surface methodology
日期:	2007
上传时间:	2009-11-13 15:25:27 (UTC+8)
摘要:	The production of spores from Bacillus amyloliquefaciens B128 was investigated in shaker-flask cultivation. Optimization of the culture medium was carried out by using a two-step approach. A quick identification of a suitable source of carbon and nitrogen was obtained by a simple screening experiment, which was followed by an application of response surface methodology (RSM) for further optimization design. A five-level four-factor central composite design was employed to determine the maximum spore yield at optimum levels for lactose, tapioca, ammonium sulfate and peptone. Tapioca and peptone showed a significant linear main effect, while lactose and ammonium sulfate had no significant linear effect. The spore production was also significantly affected by lactose-ammonium sulfate and lactose-peptone. Optimum cultivation parameters were (g/L): 12.7 of lactose, 16.7 of tapioca, 1.8 of ammonium sulfate and 8.0 of peptone. The prediction spore yield was 5.93 x 10(8) (no/mL). The actual experimental results were in agreement with the prediction. (c) 2006 Elsevier Ltd. All rights reserved.
關聯:	PROCESS BIOCHEMISTRY Volume: 42 Issue: 4 Pages: 535-541
显示于类别:	[園藝暨生物技術學系] 期刊論文

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