Optimization of the processing of porcine platelet-rich plasma and its application on human mesenchymal stem cell cultivation

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Title:	Optimization of the processing of porcine platelet-rich plasma and its application on human mesenchymal stem cell cultivation
Authors:	Lin, YK (Lin, Yung-Kai) Hsu, M (Hsu, Ma) You, WH (You, Wei-Hsun)
Contributors:	Dept Anim Sci, Biomat Lab
Keywords:	Platelet-rich plasma Mesenchymal stem cell Fetal bovine serum substitute Porcine blood utilization MSC viability MSC proliferation
Date:	2013-09-15
Issue Date:	2014-02-24 15:58:46 (UTC+8)
Abstract:	Because of ethical and scientific controversy, the utilization of fetal bovine serum (FBS) for cell culture medium must be minimized. This study develops porcine platelet-rich plasma (P-PRP) as a FBS substitute for human mesenchymal stem cell (hMSC) cultivation. Concentrating porcine blood by serial centrifugation to obtain P-PRP leads to activation by different agonist combinations to stimulate the secretion of growth factors. The concentration of growth factor in P-PRP is significantly increased by activation (p < 0.05). The concentration of PDGF, KGF and TGF-beta in activated P-PRP is significantly higher than that in FBS. Design-expert was used to decide whether Co-T+Ca, Co+T-Ca, and Co+T+Ca- are optimal agonist formulations. MSC cultivation shows that the attachment rate, proliferation rate and viability of P-PRP supplemented media are significantly higher than those for FBS-supplemented and commercial media (p < 0.05). The results demonstrate that P-PRP is an optimal FBS substitute that supports in vitro h-MSCs expansion for subsequent biomedical applications. (C) 2013 Elsevier B.V. All rights reserved.
Relation:	BIOCHEMICAL ENGINEERING JOURNAL Volume: 78 Special Issue: SI Pages: 154-162
Appears in Collections:	[Department of Animal Science] journal articles

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