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請使用永久網址來引用或連結此文件:
https://irlib.pccu.edu.tw/handle/987654321/26670
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| 題名: | Cloning, overexpression, purification and crystallization of malate dehydrogenase from Thermus thermophilus |
| 作者: | Chang, YY (Chang, Yu-Yung)
Hung, CH (Hung, Chih-Hung)
Hwang, TS (Hwang, Tzann-Shun)
Hsu, CH (Hsu, Chun-Hua) |
| 貢獻者: | Grad Inst Biotechnol |
| 日期: | 2013-11 |
| 上傳時間: | 2014-02-24 11:11:39 (UTC+8) |
| 摘要: | Malate dehydrogenase (MDH) has been used as a conjugate for enzyme immunoassay of a wide variety of compounds, such as drugs of abuse, drugs used in repetitive therapeutic application and hormones. In consideration of the various biotechnological applications of MDH, investigations of MDH from Thermus thermophilus were carried out to further understand the properties of this enzyme. The DNA fragment containing the open reading frame of mdh was amplified from the genomic DNA of T. thermophilus and cloned into the expression vector pET21b(+). The protein was expressed in a soluble form in Escherichia coli strain BL21(DE3). Homogeneous protein was obtained using a three-step procedure consisting of thermal treatment, Ni2+-chelating chromatography and size-exclusion chromatography. The purified MDH was crystallized and the crystals diffracted to a resolution of 1.80 angstrom on the BL13C1 beamline of the National Synchrotron Radiation Research Center (NSRRC), Taiwan. The crystals belonged to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 71.3, b = 86.1, c = 118.2 angstrom. The unit-cell volume of the crystal is compatible with the presence of two monomers in the asymmetric unit, with a corresponding Matthews coefficient V-M of 2.52 angstrom(3) Da(-1) and a solvent content of 51.2%. The crystal structure of MDH has been solved by molecular replacement and is currently under refinement. |
| 關聯: | ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS Volume: 69 Pages: 1249-1251 Part: 11 |
| 顯示於類別: | [生物科技研究所 ] 期刊論文
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