層接式固定聚乙二胺樹枝狀聚合物於金電極上應用於偵測雙酚A

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Title:	層接式固定聚乙二胺樹枝狀聚合物於金電極上應用於偵測雙酚A Detection of bisphenol A using layer-by-layer anchoring of polyamidoamine dendrimer to gold electrodes
Authors:	曾家葳
Contributors:	化學系應用化學碩士班
Keywords:	雙酚A 聚乙二胺樹枝狀聚合物金電極共價鍵穩定性 Bisphenol A PAMAM dendrimer gold electrode covalently bonding stability
Date:	2013
Issue Date:	2014-01-09 14:43:24 (UTC+8)
Abstract:	開發新的製程技術製備電化學感測器，偵測水中雙酚A(bisphenol A; BPA)。利用層接式(layer-by-layer)技術結合醯胺鍵結共價接合聚乙二胺樹枝狀聚合物(polyamidoamine dendrimer; PAMAM)在石英晶體金電極基板上。首先將金電極鍵結上3-mercaptopropionic acid (MPA)，再將generation 5 amine-terminated PAMAM dendrimer (G5-NH2) 藉由N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC)作為醯胺鍵偶合試劑，將聚乙二胺樹枝狀聚合物共價鍵結合於石英晶體金電極基板上。利用原子力顯微鏡（AFM）和電化學阻抗譜（EIS）檢視聚乙二胺樹枝狀聚合物共價鍵結合於石英晶體金電極基板上感測薄膜的特性。同時探討感測薄膜內層，PAMAM外層胺基數量及濃度對BPA的感測特性進行研究。最後探討以G5-NH2-MPA/Au修飾電極的最佳化製備的條件因素。在以最佳化製備條件下，G5-NH2-MPA/Au電化學感測電極對於BPA有高的感度，具有可接受範圍的檢量線、偵測極限低、快速的測試時間以及良好的長期穩定性。 Novel amperometric bisphenol A (BPA) sensors were fabricated by layer-by-layer covalently bonding polyamidoamine (PAMAM) dendrimers onto the gold electrode on quartz crystal substrate using a peptide chemical protocol. Au electrodes was pretreated with 3-mercaptopropionic acid (MPA) and further reacted with generation 5 amine-terminated PAMAM dendrimer (G5-NH2) using N-(3-dimethylaminopropyl)-N-ethylcarbodiimide hydrochloride (EDC) as peptide coupling reagent. The prepared BPA-sensing film was characterized by atomic force microscopy (AFM) and electrochemical impedance spectroscopy (EIS). The effects of the intermediate layer, the generations (sizes) of PAMAM dendrimer and the concentration of PAMAM dendrimer on the BPA sensing properties (response) of the sensors were investigated to optimize the fabrication of the modified electrodes. Additionally, factors influencing the detection processes were optimized. The BPA sensor based on G5-NH2-MPA/Au electrode exhibited a high response, an acceptable linearity, low detection limit and good long-term stability.
Appears in Collections:	[Department of Chemistry & Graduate Institute of Applied Chemistry ] thesis

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