Genetic variation of Zoysia as revealed by random amplified polymorphic DNA (RAPD) and isozyme pattern

CCUR > College of Environmental Planning & Bioresources > College of Agriculture > Graduate Institute of Biotechnology > journal articles > Item 987654321/2639

Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/2639

Title:	Genetic variation of Zoysia as revealed by random amplified polymorphic DNA (RAPD) and isozyme pattern
Authors:	Weng, Jen-Hsien Fan, Ming-Jen Lin, Chun-Yuan Liu, Ya-Hsin Hwang, Shin-Yin
Contributors:	生科所
Keywords:	genetic variation geographic distribution isozym RAPD Zoysia
Date:	2007
Issue Date:	2009-11-11 15:11:18 (UTC+8)
Abstract:	One hundred and thirty one clones of Zoysia spp. collected from 59 sites on the coasts of Taiwan and its neighboring islets were analyzed using both random amplified polymorphic DNA (RAPD) and isozymes to reveal their genetic variation. In the RAPD analysis with 12 primers, a total of 92 polymorphic bands which could distinguish 131 genotypes were found. There were 19 polymorphic bands and 81 zymogram patterns for esterase, and 9 bands and 10 patterns for acid phosphatase, respectively. Cluster analysis by the unweighted pair-group method with arithmetic means (UPGMA) of RAPD data indicated that clones collected from the same geographic region were clustered together. However, isozyme data showed discordant patterns. Interestingly, both RAPD data and isozyme fingerprinting revealed less correlation with the intuitive taxonomic classification of tested clones, but more related to the specific adaptation to the geographic or geologic aspects of their habitats.
Relation:	PLANT PRODUCTION SCIENCE Volume: 10 Issue: 1 Pages: 80-85
Appears in Collections:	[Graduate Institute of Biotechnology ] journal articles

Files in This Item:

File	Description	Size	Format
index.html		0Kb	Text	538	View/Open