| 摘要: | 許多研究指出維生素A酸 (Retinoic acid, RA)在神經發育中扮演重要的角色，維生素A酸是維生素A的代謝物，維生素A酸可調節神經的發育和功能以及細胞的分化和增生，透過兩種核受體，Retinoic acid receptors (RAR)和Retinoid X receptors (RXR)在不同的細胞會有不同的表現。半乳糖凝集素1 (Galectin-1, Gal1)，是一種Galectin，它可與β-galactosides結合，是一種β-galactosyl binding lectin， Galectin-1可以促進細胞的成長、分化、吸附，亦能調節細胞的增殖。奈米金 (Gold nanoparticles, AuNPs)，一個比較新穎的物質，有很好的生物相容性，已被運用於生物醫學上。有研究指出奈米金能調節細胞功能，促進細胞分化。本論文使用的細胞是小鼠神經瘤母細胞 (N2A cell)，將其分為四組，除控制組外分別加入維生素A酸 (10 M)、半乳糖凝集素1 (200 ng/ml)和奈米金 (5 ppm)。接下來的實驗分為兩部分。第一部分是用利用顯微鏡和Micrometrics SE Premium來觀察、照相並測量神經突觸的長度。第二部分是用MTT來測各組加藥後的細胞存活率，mitochondrial function mass來測細胞粒線體的功能，以及用QPCR來分析各組的基因表現量。結果顯示，維生素A酸、半乳糖凝集素1或奈米金促進神經瘤母細胞的突觸生長和明顯的增加突觸長度。結果顯示RA、GAL-1和AuNPs皆會顯著促使N2A細胞的神經突觸生長，而神經突觸生長是神經分化的指標；因此藉由本實驗結果可知RA、GAL-1和AuNPs可能具有潛在的神經修復能力。
Many studies suggest that Retinoic acid (RA) plays an important role in neural development. RA is a product of Vitamine A metabolism, which controls physiological processes including development, nervous system function, cell proliferation and differentiation, through the two nuclear receptors, retinoic acid receptors (RAR) and retinoid X receptors (RXR). Galectin-1 (GAL-1) is one of galectin family, GAL-1 is a β-galactosyl binding lectin, which can bind with β-galactosides. GAL-1 can promote cell growth, differentiation, adhesion, and also participates in regulating cell proliferation. Gold nanoparticles (AuNPs) a novel agent, has good biocompatibility and has been used in biomedical researches. Investigations suggest AuNPs can regulate cell function, promote cell differentiation. In this study, N2A cells (neuroblastoma cells), were divided into control, RA (10 M), GAL-1 (200 ng/ml) and AuNPs (5 ppm), respectively. Experiments were divided into two parts. First, we use microscope and Micrometrics SE Premium to observe neurite outgrowth, by taking photograph and meansuring length of axon. Secondly, this study used 3-(4,5-dimetnyl-thiazol-2-yl)2, 5-diphenyl tetrazolium bromide (MTT), real-time quantitative polymerase chain reaction (QPCR) and mitochondrial function mass to analysis cell viability, mitochondrial function and gene expression. Our results show that RA, GAL-1 and AuNPs all promote neurite outgrowth of N2A cells and significantly increase the neurite length. The results show that N2A cell neurite outgrowth is better than control group with RA, GAL-1 and AuNPs treat. Neurite outgrowth can be used as the index of neuronal differentiation. Our results show that RA, GAL-1 and AuNPs may also have neurite repair potential. |
