Effects of dehydroepiandrosterone on aldosterone release in rat zona glomerulosa cells

CCUR > College of Natural Science and Engineering > College of Engineering > Department of Chemical & Materials Engineering > journal articles > Item 987654321/2560

Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/2560

Title:	Effects of dehydroepiandrosterone on aldosterone release in rat zona glomerulosa cells
Authors:	Chang, Ling-Ling Wun, Wan-Song Alfred Wang, Paulus S.
Contributors:	化材系
Keywords:	cAMP DHEA Ca2+ steroidogenesis zona glomerulosa cells
Date:	2008
Issue Date:	2009-11-06 14:12:56 (UTC+8)
Abstract:	The present study was to investigate the effects and action mechanisms of dehydroepiandrosterone (DHEA) on steroidogenesis in rat adrenal zona glomerulosa cells (ZG). ZG cells were incubated with DHEA in the presence or absence of angiotensin II (AngII), a high concentration of potassium, 8-Br-cAMP, forskolin, 25-OH-cholesterol, pregnenolone, progesterone, deoxycorticosterone, corticosterone, A23187, or cyclopiazonic acid (CPA) at 37 degrees C for 1 h. The concentration of aldosterone or pregnenolone in the culture medium was then measured by radioimmunoassay (RIA). The cells were used to determine the cellular cAMP content. The data demonstrated that: (1) DHEA inhibited AngII-, high concentration of KCl-, forskolin-, 8-Br-cAMP-, 25-OH-cholesterol-, pregnenolone-, progesterone-, deoxycorticosterone-, corticosterone-, A23187-, or CPA-stimulated aldosterone release; (2) DHEA increased 25-OH-cholesterol-stimulated pregnenolone release but not when 25-OH-cholesterol was combined with trilostane; (3) DHEA noncompetitively inhibited aldosterone synthase but showed uncompetitive inhibition of P450scc. These results suggest that DHEA acts directly on rat ZG cells to diminish aldosterone secretion by inhibition of a post-cAMP pathway or by acting on intracellular Ca2+ mobilization. In addition it affects the function of post-P450scc steroidogenic enzymes.
Relation:	JOURNAL OF BIOMEDICAL SCIENCE Volume: 15 Issue: 4 Pages: 463-470
Appears in Collections:	[Department of Chemical & Materials Engineering] journal articles

Files in This Item:

File	Size	Format
index.html	0Kb	HTML	638	View/Open

Loading...