| 摘要: | 蛋白質體是基因體補體的表現蛋白，而蛋白質體學則是在蛋白質水準上的功能性基因體學。本研究採用蛋白質體學策略檢測和比較優秀短跑選手、長跑選手和未受訓練之對照組之間的血清蛋白質。目的：鑑定短跑與長跑選手之特異性血清蛋白質及比較兩種不同類型的徑賽運動員之間血清蛋白質體的差異。方法：利用SDS-PAGE進行血清蛋白質體分析，再透過質譜偵測法定量血清蛋白質之表現，所得數據以獨立樣本單因子變異數分析考驗組間蛋白質表現之差異，再以Scheffe法進行事後比較，顯著水準設為α=.05。結果：一、定量出307項的蛋白質，其中有26項蛋白質僅於短跑選手偵測出，18項蛋白質僅表現於長跑選手 偵測出；二、長跑選手有7項血液功能蛋白質包括玻璃連接蛋白、血清巴拉松酶1、醣蛋白fibulin-1、補體C3、維生素K依賴性蛋白、α-間胰蛋白抑制劑重鏈H3以及溫韋伯氏因子的表現量顯著低於短跑選手 (p<.05)；三、長跑選手在抗氧化功能的α白蛋白與穀胱甘過氧化酶3表現量顯著低於短跑選手 (p<.05)；四、長跑選手有7項免疫功能蛋白質包括γ-3鏈C區免疫球蛋白，類多肽免疫球蛋白5、群集素、補體C1s組成分、補體因子B、補體C4-A以及補體C1q組成分次單元A的表現量顯著低於短跑選手 (p<.05)。結論：本研究明確的區別出優秀男子短跑和長跑運動員血清蛋白質體學的特性及潛在的生物標記，並發現短跑與長跑不同型態的運動可能會造成凝血作用、抗氧化能力及免疫調節等血清蛋白質表現量的差異性。
Proteomics is the performance of genomic complement proteins, and proteomics is the protein level on functional genomics. In this study, proteomic strategies to detect and relatively elite sprinter (sprint runner group, SR), long distance runners (long-distance runner group, LDR) and theuntrainedcontrol group (control group, CON) between serum proteins. Purposes: To identify the sprint and long distance runners marathon specific serum protein and comparison of two different types of track athletes difference between the serum proteome. Methods: Serum protein fractionations were separated by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and then analyzed by a quantitative nano-LC-MS/MS-based proteomic profiling. The one-way analysis of variance (ANOVA) followed by Scheffe post hoc comparison was used to determine whether there is any significant difference of each protein level between three groups, the significance level (alpha value) was set at .05. Results: (1) Of the 307 identified proteins, there were 26 unique proteins in the SR group, and 18 unique proteins in the LDR group. (2) The expression levels of 7 coagulation function-associated proteins (vitronectin, serum paraoxonase/arylesterase 1, fibulin-1, complement C3, vitamin K-dependent protein, inter-alpha-trypsin inhibitor heavy chain H3 and von Willebrand factor) were significantly decreased in the group LDR compared to group SR. (3) The expression levels of 2antioxidantproteins,afamin and glutathione peroxidase 3, were also significantly decreased in the group LDR compared to group SR. (4) The expression levels of 7 immune function-related proteins (Ig gamma-3 chain C region, Ig lambda-like polypeptide 5, clusterin, complement C1s subcomponent, complement factor B, complement C4-A, complement C1q subcomponent subunit A) were significantly decreased in the group LDR compared to group SR. Conclusion: This study results clearly identified the potential serum protein markers for elite sprinters and long distance runners. The changes in regulation of coagulation, antioxidant or immune function-specific proteins may also provide further clinical applications for these two different track athletes. |
