Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack

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Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/2443

Title:	Gene stacking in Phalaenopsis orchid enhances dual tolerance to pathogen attack
Authors:	Chan, Y.L.;Lin, K.H.;Sanjaya;Liao, L.J.;Chen, W.H.;Chan, M.T.
Contributors:	園藝學系
Date:	2005
Issue Date:	2009-10-30 15:49:35 (UTC+8)
Abstract:	Cymbidium Mosaic Virus (CymMV) and Erwinia carotovora have been reported to cause severe damage to orchid plants. To enhance the resistance of orchids to both viral and bacterial phytopathogens, gene stacking was applied on Phalaenopsis orchid by double transformation. PLBs originally transformed with CymMV coat protein cDNA (CP) were then re-transformed with sweet pepper ferredoxin-like protein cDNA (Pflp) by Agrobacterium tumefaciens, to enable expression of dual (viral and bacterial) disease resistant traits. A non-antibiotic selection procedure in the second transformation minimized the potential rate of 'stacking' antibiotic genes in the orchid gene pool. Transgene integration in transgenic Phalaenopsis lines was confirmed by Southern blot analysis for both CP and pflp genes. Expression of transgenes was detected by northern blot analysis, and disease resistant assays revealed that transgenic lines exhibited enhanced resistance to CymMV and E. carotovora. This is the first report describing a transgenic Phalaenopsis orchid with dual resistance to phytopathogens.
Relation:	TRANSGENIC RESEARCH v.14 n.3 Pages: 279-288
Appears in Collections:	[Department of Horticulture] journal articles

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