球根花卉健康種苗繁殖-海芋試管內植株再生途徑之研究

文化大學機構典藏 CCUR > 環境規劃暨生物資源學院 > 農學院 > 園藝暨生物技術學系 > 研究計畫 > Item 987654321/24390

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题名:	球根花卉健康種苗繁殖-海芋試管內植株再生途徑之研究 Studies on healthy plantlet propagation of flower bulb crops- in vitro plant regeneration of Zantedeschia aethiopica
作者:	陳麗如黃子彬熊同銓林冠宏陳昱宇
贡献者:	園藝暨生物技術學系
关键词:	海芋種苗器官發生體胚發生植物再生球根花卉
日期:	2011-2013
上传时间:	2013-03-01 11:01:18 (UTC+8)
摘要:	建立海芋試管內植株再生途徑以培育及生產健康種苗，是海芋產業永續發展的重要課題。現已完成白花海芋 (<EM>Zantedeschia aethiopica</EM> L.) 無菌消毒條件的建立、體胚誘導及直接芽體器官發生途徑。以5％ H<SUB>2</SUB>O<SUB>2</SUB>處理後接續NaOCl消毒，可降低瓶內污染率。切取白花海芋根莖含形成層的組織，培養於添加1 mg l<SUP>-1</SUP> 2,4-D及6％ sucrose的MS基本培養基中，體胚誘導率為33％。切取帶主芽的根莖培植體，於添加1 mg l<SUP>-1</SUP> 2,4-D的MS基本培養基中，可顯著促進芽體的生長，切取其芽基盤培養於MS基本培養基中，可達3.5倍的芽體增殖率，分切後均可發育為完整植株。目前已建立白花海芋芽體器官發生途徑並誘導體胚形成，本計畫將接續探討促進試管內根莖種球形成的條件，未來將可開發試管內植株再生途徑以生產健康種苗及種球，並可作為基因轉殖的平台，以利分子育種的研究。 The mass production of healthy rhizome bulbs of <EM>Zantedeschia aethiopica</EM> L. through <EM>in vitro</EM> micorpropation is an important issue for a sustainable development of calla lily industry in Taiwan. The aim of this study is to establish<EM> in vitro</EM> plant regeneration of white calla lily. Aseptic sterilization, somatic embryogenesis and direct organogenesis in<EM> Z. aethiopica</EM> L. have been established in this study. Pretreatment with 5％ H<SUB>2</SUB>O<SUB>2</SUB> for 5 min followed with 1％ NaOCl for 15 min can decrease <I>in vitro</I> contamination. Supplemented with 1 mg l<SUP>-1</SUP> 2,4-D and 6％ sucrose in Murashige and Skoog (MS) basal medium can induce somatic embryo formation (33.3％) on rhizome explants with cambium in<EM> Z. aethiopica</EM> L. MS basal medium containing 1 mg l<SUP>-1</SUP> 2,4-D can promote the shoot/plantlet development from rhizome buds. After culture of 4 wk, the shoot basal discs were cut from plantlets and cultured in MS basal medium, in which condition shoots proliferated with 3.5 folds. All shoots can develop into healthy plantlets. We have established<EM> in vitro</EM> shoot organogenesis and induce the formation of somatic embryo. In this plan, we will further to find the optimal cultural conditions on<I> in vitro</I> bulb formation for establishing the healthy plantlet propagation of <EM>Z. aethiopica</EM> L.
显示于类别:	[園藝暨生物技術學系] 研究計畫

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