Production and Characterization of a Bacteriocin from Ruminal Bacterium Ruminococcus albus 7

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Title:	Production and Characterization of a Bacteriocin from Ruminal Bacterium Ruminococcus albus 7
Authors:	Wang, HT (Wang, Han-Tsung) Chen, IH (Chen, I-Hung) Hsu, JT (Hsu, Jih-Tay)
Contributors:	Dept Anim Sci
Keywords:	antibiotic alternative bacteriocin ruminal bacteria purification stimulation
Date:	2012-01
Issue Date:	2013-02-26 10:38:41 (UTC+8)
Abstract:	The characteristics of a bacteriocin from Ruminococus albus 7 and its potential as an antibiotic alternative were examined in this study. The addition of 3 Km 3-phenylpropanoic acid (PPA) and 0.2% Tween 80 to the culturing medium improved bacteriocin production by 2.5-fold. Native polyacrylamide gel electrophoresis of the antagonistically active gel filtration fraction established that the molecular weight of the R. albus 7 bacteriocin was approximately 36 kDa. The bacteriocin was sensitive to pepsin, protease, and pancreatin, and was inactivated by heating at 65 C for 1 h. Simulating in vitro avian digestion decreased the antagonistic activity by 74.7%, but the addition of 1% bovin serum albumin restored 13% of the lost antagonistic activity. Following ion-exchange purification, the bacteriocin had sufficient antagonistic activity against five tested pathogenic strains, but the addition of a protectant is necessary for utilization of bacteriocin of R. albus 7 as an antibiotic alternative in animal feed.
Relation:	BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 卷: 76 期: 1 頁數: 34-41
Appears in Collections:	[Department of Animal Science] journal articles

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