In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents

文化大學機構典藏 CCUR > 理工學院 > 理學院 > 生命科學系 > 期刊論文 > Item 987654321/24310

Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/24310

Title:	In vitro assessment of reproductive toxicity of cigarette smoke and deleterious consequences of maternal exposure to its constituents
Authors:	Wu, SC (Wu, Shao-Chin) Liu, M (Liu, Min)
Contributors:	Dept Life Sci
Keywords:	cigarette smoke oocyte maturation reproductive toxicity sperm capacitation
Date:	2012
Issue Date:	2013-02-25 15:02:54 (UTC+8)
Abstract:	Cigarette smoke is known to be a serious health risk factor and considered reproductively toxic. In the current study, we investigated whether constituents of cigarette smoke, pyrazine, 2-ethylpyridine, and 3-ethylpyridine, adversely affect reproductive functioning such as oocyte maturation and sperm capacitation. Our findings indicated that three smoke components were involved in retardation of oocyte maturation in a dose-dependent manner and the lowest-observed-adverse-effect level (LOAEL) was determined to be 10(-10)M. However, individual smoke components administrated at the LOAEL did not attenuate oocyte maturation, demonstrating that all three toxicants were equally required for the observed growth impairment. When exposed to all three components at 10(-10)M during in vitro capacitation, murine sperm lost forward progression and were unable to show adequate hyperactivation, which is indicative of the incompletion of the capacitation process. Only sperm administrated with 3-ethylpyridine alone showed significant reduction in capacitation status, suggesting the chemical is the one responsible for disrupting sperm capacitation. Taken together, this is the first report that documents the effect of cigarette smoke components on oocyte maturation and sperm capacitation. The present findings demonstrate the adverse effects of smoke constituents of mammalian reproduction and the differences in sensitivity to smoke components between male and female gametes. Since both processes take place in the female reproductive system, our data provide new insights into deleterious consequences of maternal exposure to cigarette smoke.
Relation:	BIOLOGICAL RESEARCH 卷: 45 期: 2 頁數: 101-109
Appears in Collections:	[生命科學系] 期刊論文

Files in This Item:

There are no files associated with this item.

View Licence

Loading...