THE CONSTRUCTION OF BIFUNCTIONAL FUSION XYLANOLYTIC ENZYMES AND THE PREDICTION OF OPTIMUM REACTION CONDITIONS FOR THE ENZYME ACTIVITY

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题名:	THE CONSTRUCTION OF BIFUNCTIONAL FUSION XYLANOLYTIC ENZYMES AND THE PREDICTION OF OPTIMUM REACTION CONDITIONS FOR THE ENZYME ACTIVITY
作者:	Pai, CK (Pai, Cheng-Kang) Wang, HT (Wang, Han-Tsung) Guo, RT (Guo, Rey-Ting) Liu, JR (Liu, Je-Ruei)
贡献者:	Dept Anim Sci
关键词:	Chimeric enzyme Neocallimastix patriciarum Xylanase Acetylxylan esterase
日期:	2012
上传时间:	2013-02-25 14:23:01 (UTC+8)
摘要:	Four chimeric xylanolytic enzymes were formed by fusion of a thermally stable xylanase XynCDBFV either to the N-terminus or C-terminus of a thermally stable acetylxylan esterase AxeS20E, with or without a Gly-rich flexible linker (S2). The three-dimensional (3D) structures of the chimeric enzymes were predicted using the I-TASSER server, and the results indicated that the structures of Axe-S2-Xyn and Xyn-S2-Axe were more similar to the native structures than were those of Axe-Xyn and Xyn-Axe. Axe-S2-Xyn and Xyn-S2-Axe were expressed in Escherichia coli and purified by means of affinity chromatography. Response surface modeling (RSM), combined with central composite design (CCD) and regression analysis, was then employed to optimize the xylanase activities of the chimeric enzymes. Under the optimal conditions, Xyn-S2Axe had greater hydrolytic activities on natural xylans and rice straw than did the parental enzymes. These results suggested that the chimeric enzyme Xyn-S2-Axe could be effective at hydrolyzing xylan in biomass and that it has potential to be used in a range of biotechnological applications.
關聯:	BIORESOURCES 卷: 7 期: 4 頁數: 5647-5665
显示于类别:	[Department of Animal Science] journal articles

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