文化大學機構典藏 CCUR:Item 987654321/24285
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 46962/50828 (92%)
造访人次 : 12440582      在线人数 : 583
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于CCUR ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/24285


    题名: Role of p21 as a determinant of 1,6-Bis[4-(4-amino-3-hydroxyphenoxy)phenyl] diamantane response in human HCT-116 colon carcinoma cells
    作者: Wang, JJ (Wang, Jane-Jen)
    Hung, HF (Hung, Hui-Fang)
    Huang, ML (Huang, Min-Li)
    Lee, HJ (Lee, Hui-Ju)
    Chern, YT (Chern, Yaw-Terng)
    Chang, YF (Chang, Yuh-Fang)
    Chi, CW (Chi, Chin-Wen)
    Hsu, YC (Hsu, Yi-Chiung)
    贡献者: Dept Hort & Biotechnol
    关键词: colorectal carcinoma
    cell cycle
    p21
    anti-proliferation
    anti-migration
    diamantane
    日期: 2012-03
    上传时间: 2013-02-22 16:16:36 (UTC+8)
    摘要: 1,6-Bis[4-(4-amino-3-hydroxyphenoxy)phenyl] diamantane (DPD) induces growth inhibition in human cancer cells. In our previous study, we discovered that DPD irreversibly inhibits the growth of Colo 205 colon cancer cells at the G(0)/G(1) phase and induces cell differentiation. However, the detailed mechanism is still unknown. In this study, we examined the functional importance of p21 and p53 in DPD-induced anticancer effects. We used three isogenic cell lines, HCT-116, HCT-116 p53(-/-) and HCT-116 p21(-/-), to evaluate the roles of p21 and p53 in the in vitro anticancer effects of DPD. The in vivo anti-proliferative effect of DPD was demonstrated by HCT-116 and HCT-116 p21(-/-) xenograft models. DPD significantly inhibited the growth as well as increased the number of HCT-116 cells in the G(0)/G(1), phase, but not in HCT-116 p53(-/-) and HCT-116 p21 cells examined by flow cytometry. Additionally, Western blot analysis showed that DPD treatment induced p21, but not p53 protein expression in HCT-116 cells. The p21-associated cell cycle regulated proteins, such as cyclin D, CDK4 and pRb were decreased after DPD treatment in HCT-116 cells. The DPD-increased G(0)/G(1), phase and induced cell cycle regulated protein expression were not observed in HCT-116 p21(-/-) and HCT-116 p53(-/-) cells. DPD decreased cell migration in HCT-116 and HCT-116 p53(-/-) but not in HCT-116 p21(-/-) cells. p21 was required for the DPD-induced in vitro anti-colon cancer effect. The in vivo study also showed that DPD significantly inhibited tumor growth through p21 signaling. Our results clearly demonstrate that DPD-induced in vitro and in vivo anticancer effects through the activation of p21 in HCT-116 cells.
    關聯: ONCOLOGY REPORTS 卷: 27 期: 2 頁數: 529-534
    显示于类别:[園藝暨生物技術學系] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html0KbHTML391检视/开启


    检视Licence

    在CCUR中所有的数据项都受到原著作权保护.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈