文化大學機構典藏 CCUR:Item 987654321/24221
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/24221


    题名: Beta-adrenoceptor pathway enhances mitochondrial function in human neural stem cells via rotary cell culture system
    作者: Chiang, MC (Chiang, Ming-Chang)
    Lin, H (Lin, Heng)
    Cheng, YC (Cheng, Yi-Chuan)
    Yen, CH (Yen, Chia-Hui)
    Huang, RN (Huang, Rong-Nan)
    Lin, KH (Lin, Kuan-Hung)
    贡献者: Grad Inst Biotechnol
    关键词: Rotary cell culture system
    Human neural stem cells
    PGC1 alpha
    Mitochondrial function
    日期: 2012-06
    上传时间: 2013-02-20 14:43:04 (UTC+8)
    摘要: The structure and function of the human nervous system are altered in space when compared with their state on earth. To investigate directly the influence of simulated microgravity conditions which may be beneficial for cultivation and proliferation of human neural stem cells (hNSCs), the rotary cell culture system (RCCS) developed at the National Aeronautics and Space Administration (NASA) was used. RCCS allows the creation of a unique microgravity environment of low shear force, high-mass transfer and enables three-dimensional (3D) cell culture of dissimilar cell types. The results show that simulated microgravity using an RCCS would induce p-adrenoceptor, upregulate cAMP formation and activate both PKA and CREB (cAMP response element binding protein) pathways. The expression of intracellular mitochondrial genes, including PGC1 alpha (PPAR coactivator 1 alpha), nuclear respiratory factors 1 and 2 (NRF1 and NRF2) and mitochondrial transcription factor A (Tfam), regulated by CREB, were all significantly increased at 72 h after the onset of microgravity. Accordingly and importantly, the ATP level and amount of mitochondria] mass were also increased. These results suggest that exposure to simulated microgravity using an RCCS would induce cellular proliferation in hNSCs via an increased mitochondrial function. In addition, the RCCS bioreactor would support hNSCs growth, which may have the potential for cell replacement therapy in neurological disorders. (C) 2012 Elsevier B.V. All rights reserved.
    關聯: JOURNAL OF NEUROSCIENCE METHODS 卷: 207 期: 2 頁數: 130-136
    显示于类别:[生物科技研究所 ] 期刊論文

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