文化大學機構典藏 CCUR:Item 987654321/24218
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    Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/24218


    Title: Expression profiles of a MhCTR1 gene in relation to banana fruit ripening
    Authors: Hu, HL (Hu, Huei-Lin)
    Do, YY (Do, Yi-Yin)
    Huang, PL (Huang, Pung-Ling)
    Contributors: Grad Inst Biotechnol
    Keywords: CTR1
    Ethylene signaling
    Fruit ripening
    Climacteric fruit
    Musa spp.
    Date: 2012-07
    Issue Date: 2013-02-20 14:31:19 (UTC+8)
    Abstract: The banana (Musa spp.) is a typical climacteric fruit of high economic importance. The development of bananas from maturing to ripening is characterized by increased ethylene production accompanied by a respiration burst. To elucidate the signal transduction pathway involved in the ethylene regulation of banana ripening, a gene homologous to Arabidopsis CTR1 (constitutive triple response 1) was isolated from Musa spp. (Hsien Jin Chiao, AAA group) and designated as MhCTR1.MhCTR1 spans 11.5 kilobases and consists of 15 exons and 14 introns with consensus GT-AG nucleotides situated at their boundaries. MhCTR1 encodes a polypeptide of 805 amino acid residues with a calculated molecular weight of 88.6 kDa. The deduced amino acid sequence of MhCTR1 demonstrates 55%, 56% and 55% homology to AtCTR1, RhCTR1, and LeCTR1, respectively. MhCTR1 is expressed mostly in the mature green pulp and root organs. During fruit development MhCTR1 expression increases just before ethylene production rises. Moreover, MhCTR1 expression was detected mainly in the pulps at ripening stage 3, and correlated with the onset of peel yellowing, while MhCTR1 was constitutively expressed in the peels. MhCTR1 expression could be induced by ethylene treatment (0.01 mu L-1), and MhCTR1 expression decreased in both peel and pulp 24 h after treatment. Overall, changes observed in MhCTR1 expression in the pulp closely related to the regulation of the banana ripening process. (C) 2012 Published by Elsevier Masson SAS.
    Relation: PLANT PHYSIOLOGY AND BIOCHEMISTRY 卷: 56 頁數: 47-55
    Appears in Collections:[Graduate Institute of Biotechnology ] journal articles

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