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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/24192


    题名: The Role of Type III Secretion System and Lens Material on Adhesion of Pseudomonas aeruginosa to Contact Lenses
    作者: Shen, EP (Shen, Elizabeth P.)
    Tsay, RY (Tsay, Ruey-Yug)
    Chia, JS (Chia, Jean-San)
    Wu, S (Wu, Semon)
    Lee, JW (Lee, Jing-Wen)
    Hu, FR (Hu, Fung-Rong)
    贡献者: Dept Life Sci
    关键词: CORNEAL EPITHELIAL-CELLS
    MICROBIAL KERATITIS
    BACTERIAL ADHESION
    RISK-FACTORS
    EXOENZYME S
    CONVENTIONAL HYDROGEL
    TEAR CALCIUM
    VIRULENCE FACTORS
    IN-VITRO
    INFECTION
    日期: 2012-09
    上传时间: 2013-02-20 09:47:32 (UTC+8)
    摘要: PURPOSE. To determine the distribution of invasive and cytotoxic genotypes among ocular isolates of P. aeruginosa and investigate the influence of the type III secretion system (T3SS) on adhesion to conventional, cosmetic, and silicone hydrogel contact lenses (CL).

    METHODS. Clinical isolates from 2001 to 2010 were analyzed by multiplex PCR for exoS, exoU, and exoT genes. Bacterial adhesion to etafilcon, nelfilcon (gray colored), balafilcon, and galyfilcon CL with or without artificial tear fluid (ATF) incubation were compared. Surface characteristics were determined with scanning electron microscopy (SEM).

    RESULTS. Among 87 total isolates, 64 strains were from microbial keratitis cases. CL-related microbial keratitis (CLMK) isolates were mostly of the cytotoxic genotype (expressing exoU) (P = 0.002). No significant differences were found in bacterial adhesion to all types of CL between the genotypes under T3SS-inducing conditions. A trend for least bacterial adhesion of galyfilcon compared to the other CL was noted for both genotypes. Needle complex pscC mutants adhered less to all materials than the wild type (P < 0.05), indicating a role of the T3SS in contact lens adhesion. ATF-incubated CL had significantly more bacterial adhesion (P < 0.05). SEM showed most of the bacteria adhering on CL surfaces.

    CONCLUSIONS. CLMK isolates were mostly of cytotoxic genotype. Different genotypes did not significantly differ in its adhesion to various CL. T3SS and other adhesins are involved in bacteria-contact lens adhesion through complex interactions. Contact lens materials may also play an important role in the adherence of both genotypes of P. aeruginosa.
    關聯: INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE 卷: 53 期: 10 頁數: 6416-6426
    显示于类别:[生命科學系] 期刊論文

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