Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris

文化大學機構典藏 CCUR > 理工學院 > 工學院 > 化學工程與材料工程學系暨碩士班 > 期刊論文 > Item 987654321/24180

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题名:	Lower Temperature Cultures Enlarge the Effects of Vitreoscilla Hemoglobin Expression on Recombinant Pichia pastoris
作者:	Wu, JM (Wu, Jyh-Ming) Wang, SY (Wang, Shin-Yao) Fu, WC (Fu, Wei-Chang)
贡献者:	Dept Chem & Mat Engn
关键词:	Vitreoscilla hemoglobin lower temperature Pichia pastoris AOX 1 promoter recombinant protein production
日期:	2012-10
上传时间:	2013-02-19 14:22:37 (UTC+8)
摘要:	An heterologous expression of Vitreoscilla hemoglobin (VHb) for improving cell growth and recombinant protein production has been successfully demonstrated in various hosts, including Pichia pastoris. Lower temperature cultures can enhance target protein production in some studies of P. pastoris. In this study, the strategy of combining heterologous VHb expression and lower temperature cultures in P. pastoris showed that final cell density and viability of VHb(+) strain at 23 degrees C were higher than that at 30 degrees C. In addition, the effects of VHb expression on recombinant beta-galactosidase production and oxygen uptake rate were also higher at 23 degrees C than at 30 degrees C. Consequently, lower temperature cultures can enlarge VHb effectiveness on cell performance of P. pastoris. This is because VHb activity obtained at 23 degrees C cultures was twofold higher than that at 30 degrees C cultures, due to a different heme production. This strategy makes P. pastoris an excellent expression host particularly suitable for increasing the yields of the low-stability and aggregation-prone recombinant proteins.
關聯:	INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 卷: 13 期: 10 頁數: 13212-13226
显示于类别:	[化學工程與材料工程學系暨碩士班] 期刊論文

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