| 题名: | STUDY OF THE INTERACTION OF METHYLENE VIOLET WITH CALF THYMUS DNA USING AN SPR-BASED DNA SENSOR |
| 作者: | Tsai, WC (Tsai, Wen-Chi)
Lan, YC (Lan, Yun-Chun) |
| 贡献者: | Grad Inst Biotechnol |
| 关键词: | DNA biosensor
Interaction
Methylene violet
Surface plasmon resonance
Zirconia thin films |
| 日期: | 2011 |
| 上传时间: | 2013-01-31 10:22:23 (UTC+8) |
| 摘要: | Electrochemical and surface plasmon resonance technologies were combined to detect binding of low-molecular-weight compounds to DNA. First, zirconia thin films were electrochemically deposited onto bare gold electrodes. Second, calf thymus DNA was attached onto the zirconia thin films. Finally, the interaction of methylene violet with the DNA-modified surface was tested. The binding isotherm gave a K-D of 2.21 x 10(-5) M. Fluorescence quenching experiments were performed to confirm the interaction. Regenerating surfaces with 1 mM NaOH provided reusable surfaces. This study provides a generic platform which can be tailored for the study of interactions of small molecules with DNA. |
| 關聯: | ANALYTICAL LETTERS 卷: 44 期: 4 頁數: 717-726 |
| 显示于类别: | [生物科技研究所 ] 期刊論文
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