Extraction and characterisation of telopeptide-poor collagen from porcine lung

文化大學機構典藏 CCUR > 環境規劃暨生物資源學院 > 農學院 > 動物科學系 > 期刊論文 > Item 987654321/24078

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/24078

题名:	Extraction and characterisation of telopeptide-poor collagen from porcine lung
作者:	Lin, YK (Lin, Yung-Kai) Lin, TY (Lin, Tung-Yung) Su, HP (Su, Hou-Pin)
贡献者:	Dept Anim Sci
关键词:	Porcine lung Telopeptide-poor collagen Optimum extraction condition
日期:	2011-02-15
上传时间:	2013-01-25 10:11:44 (UTC+8)
摘要:	The aim of the investigation was to develop optimum extraction conditions of telopeptide-poor collagen from porcine lung (TPCPL). Chemical composition, histological properties of porcine lung, removal efficiency of haemoglobin by different solvents and effects of pepsin solubilisation time on TPCPL properties were evaluated. Histological observation of lung tissue revealed collagen coexists with elastin. Treatment with 0.1 M Tris-HCl/5% Triton X-100 (pH 8.2) for 12 h exhibited the highest efficiency for removal of haemoglobin. The maximal yield of TPCPL reached 6.702 g/kg by pepsin solubilisation for 72 h. SDS-PAGE indicated that type I collagen was a major component of TPCPL: FTIR spectra of all TPCPL were similar to commercial porcine collagen and absent from denatured product (gelatin) and TPCPL 72 exhibited the lowest denaturation temperature (36.4 degrees C) and maximum enzymatic sensitivity. Finally, the optimum pepsin solubilisation of TPCPL was controlled at 4 degrees C for 48 h for better structural integrity and acceptable yield. (C) 2010 Elsevier Ltd. All rights reserved.
關聯:	FOOD CHEMISTRY 卷: 124 期: 4 頁數: 1583-1588
显示于类别:	[動物科學系 ] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	506	检视/开启

检视Licence

在CCUR中所有的数据项都受到原著作权保护.

数据加载中.....