文化大學機構典藏 CCUR:Item 987654321/24039
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 47126/50992 (92%)
造访人次 : 13865272      在线人数 : 284
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于CCUR ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/24039


    题名: Identification of SEC61 beta and its autoantibody as biomarkers for colorectal cancer
    作者: Fan, CW (Fan, Chung-Wei)
    Chan, CC (Chan, Chung-Chuan)
    Chen, KT (Chen, Kuei-Tien)
    Twu, J (Twu, Jen)
    Huang, YS (Huang, Ya-Shu)
    Han, CL (Han, Chia-Li)
    Chen, YJ (Chen, Yu-Ju)
    Yu, JS (Yu, Jau-Song)
    Chang, YS (Chang, Yu-Sun)
    Kuo, YB (Kuo, Yung-Bin)
    Chan, EC (Chan, Err-Cheng)
    贡献者: Dept Chem
    关键词: Autoantibody
    Colorectal cancer
    SEC61 beta
    Serological biomarker
    日期: 2011-05-12
    上传时间: 2013-01-18 10:58:50 (UTC+8)
    摘要: Background: To identify novel serological biomarkers for human colorectal cancer (CRC). we analyzed CRC tissues using gel-assisted digestion and isobaric tags with related and absolute quantitation (iTRAQ) labeling mass spectrometry (MS). By comparing pairs of tumor tissues and matched normal tissues. we discovered the SEC61 beta with expression changes 3.3-fold and a marginal statistical significance (p=0.052) previously.

    Methods: SEC61 beta expression in CRC tissues was further analyzed by western blotting and immunohistochemistry. We next assessed the putative diagnostic value of the SEC61 beta autoantibody as a serum marker.

    Results: Using western blotting analysis. SEC61 beta expression was increased 1.9-fold in tumor tissues. Immunohistochemical analysis of 64 CRC specimens showed that SEC61 beta was positively detected in 64% of the tumors, but weakly or not detected in > 80% of the adjacent nontumor epithelial cells. Western blot analysis with plasma samples showed that the sensitivity and specificity of the SEC61 beta autoantibody from patients with CRC were 79% and 75%. respectively. Importantly, the results of the SEC61 beta autoantibody for early detection of colorectal cancer revealed a higher sensitivity of 77% than the carcinoembryonic antigen (CEA) assay.

    Conclusions: Measurement of SEC61 beta autoantibody levels may provide an alternative detection indicator for CRC, particularly among early-stage patients. (C) 2011 Elsevier B.V. All rights reserved.
    關聯: CLINICA CHIMICA ACTA Volume: 412 Issue: 11-12 Pages: 887-893
    显示于类别:[化學系所] 期刊論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html0KbHTML333检视/开启


    检视Licence

    在CCUR中所有的数据项都受到原著作权保护.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈