共軛亞麻油酸（CLA）具多種健康功能文獻已有詳載，而應用光照射處理技術生產富含共軛亞麻油酸之紅花籽油極具潛力，然而，目前對於應用光反應技術生產共軛亞麻油酸之研究報告為數不多，因此本研究應用光反應技術，在不同光照溫度與時間下生產共軛亞麻油酸，以探討光反應條件對於促進共軛亞麻油酸生產之影響。
材料選用目前市售沙拉油中含最高量亞麻油酸之紅花籽油，在添加I2作為催化劑後，以3650Å UV光線進行光催化合成反應，溫度設定為25℃與75℃，並於0、48、96、144、192與240小時採樣，其後進行游離態共軛亞麻油酸與甘油酯化態共軛亞麻油酸甲基酯化反應，最後使用高效液相層析儀進行共軛亞麻油酸分析。
結果發現：光照溫度不同會改變共軛亞麻油酸生成種類與生成量，經25℃光反應生成共軛亞麻油酸結構為游離態共軛亞麻油酸，且共軛亞麻油酸生成量較75℃為佳；光反應合成cis-9, trans-11與trans-10, cis-12 CLA異構物之最佳光照條件為25℃、144小時，而25℃、240小時光照條件生成之總共軛亞麻油酸與總trans, trans CLA異構物含量最高。
Conjugated linoleic acid (CLA) had been reported to be responsible for many biological activities that relate to health. There is a potential on CLA production using photoirradiation on safflower oil. However, only a few Reports on CLA production using photoirradiation were found. The objective of the study is to investigate the effects of photoirradiation of different temperatures and times on CLA produced from safflower oil.Safflower oil was photoirradiated under 3650Å UV-radiation at 25 and 75℃in the presence of iodine catalyst for 240 h and was withdrawn at intervals of 0, 48, 96, 144, 192, and 240 h for methylation and CLA determination by HPLC. Results indicated that photoirradiation temperature could alter the variety and content of CLA produced from safflower oil. Irradiation temperature at 25℃ produced free form of CLA, rather than CLA glyceride, and the level of CLA produced was higher at 25 than 75℃. While photoirradiation condition at 25℃ for 144 h was suggested for the productions of cis-9, trans-11 and trans-10, cis-12 CLA from safflower oil. Condition at 25℃ for 240 h was suggested for the production of
trans, trans CLA.
