The research with Lyocell by caustic treatment and enzyme treatment, probe into the change of Zeta Potential. Lyocell make the swollen phenomenon by caustic treatment. Lyocell make with enzyme treatment. Cellulose of fiber surface make to decompose by enzyme treatment, produce to destroy on surface. through caustic treatment and enzyme treatment fiber make to 25% caustic treatment and enzyme treatment, Zeta Potential to differentiate -17.73mv、-28.25mv、-31.49mv, to find Zeta Potential with add. then all right inference, fiber make the swollen phenomenon, and produce to destroy on surface, Zeta Potential will be obviously received and changed.