This experiment is trying to get the physiological variation of human body in static state and dynamic state with cool fabrics and cotton fabrics. The differences of heating, Autonomic Nervous System, and surface temperatures of human body are measured when people wear various fabrics. As a result, the thermal effect of cool fabrics is obtained. Thus, the percentage of COOL NYLON and the weaving of cool fabrics are changed to create the functional and low-cost cool fabrics.