The study on polypropylene (abbri. PP) nonwoven were grafted with acrylic acid used benzoyl peroxide (BPO) as initiator, and then immobilized with collagen and Rhizochitosan about 80% deacetylation onto modified PP nonwovens. The effect of mixing ratio of collagen/Rhizochitosan on immobilizing ratio and anti-bacterial properties of modified PP nonwoven fabrics had deeply been investigated. From experimental result shown that the P.P.nonwoven grafted with acrylic acid about 15% and ratio of collagen/Rhizochitosan at 1:2, immobilized on the modified PP for 18hrs, could obtain good immobilizing results. On the other hand, the inhibition zone increase with increasing the Rhizochitosanratio.