文化大學機構典藏 CCUR:Item 987654321/20964
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    题名: Quantitative trait loci influencing fruit-related characteristics of tomato grown in high-temperature conditions
    作者: Lin, KH (Lin, Kuan-Hung)
    Yeh, WL (Yeh, Wei-Lung)
    Chen, HM (Chen, Huei-Mei)
    Lo, HF (Lo, Hsiao-Feng)
    贡献者: 生科所
    关键词: Tomato
    Quantitative trait loci (QTLs)
    Marker-assisted selection (MAS)
    Random amplified polymorphic DNA (RAPD)
    Inter simple sequence repeat (ISSR)
    Amplified fragment length polymorphism (AFLP)
    日期: 2010-07
    上传时间: 2011-12-09 15:34:08 (UTC+8)
    摘要: The objective of this study was to identify molecular markers linked to fruit-related traits in the tomato subjected to high temperatures. In total, 160 F(2) plants derived from a cross between a heat-tolerant breeding line, CL5915-93D4-1-0-3 (Solanum esculentum), and a heat-sensitive wild accession, L4422 (S. pimpinellifolium), were grown in a greenhouse. Six traits including fruit number, fruit weight, brix, seed number, fruit setting, and flower number were scored. The distributions of fruit number, fruit set, flower number, and seed number were skewed towards heat susceptibility which is known to be characteristic of L4422. Polymorphic bands were generated by PCR-derived methods of RAPD, ISSR and AFLP Polymorphism, the segregation ratio, and distribution over the genome of the above 3 markers were compared. Ten linkage groups, ranging 20.6-151.6 cM in size, were constructed with 62 informative markers spanning a total of 776.3 cM. Fruit-related quantitative trait loci (QTLs) were non-randomly distributed in the tomato genome. For the 6 traits investigated, 21 QTLs were dispersed on linkage groups 2-5. The genetic effects of the various QTLs were differently exhibited, in our study we have respectively found from 10.5% to 30.2% of the variation explained by the QTL for flower number (FRN4) and brix (BX2). Thirteen QTL-mapped markers were unique to 1 trait, and 4 markers were linked to more than 1 trait. Among them, QTLs linked to the I868-470 marker had effects on fruit weight and brix, and a significant positive correlation between these 2 traits was noted (r = 0.35, P < 0.05). Thus, the I868-470 marker may have the potential for simultaneous selection of high fruit weight and brix. These markers also allowed us to align genome linkage maps across distantly related species and to reveal the co-localization between these QTLs and major genes.
    显示于类别:[生物科技研究所 ] 期刊論文

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