The wound dressing of acrylic acid-grafted and chitosan/collagen-immobilized polypropylene non-woven fabric (PP-AAg-CCi) were produced. In this study, two kinds of chitosan obtained from the nourishment of Mucor (m-chitosan) and from commerce (c-chitosan) were used for comparison. It was found that the values of water absorbing and water diffusion coefficient for m-chitosan sample (PP-AAg-CmCi) were significantly higher than that for c-chitosan sample (PP-AAg-CcCi). The enhanced percentage oil water absorbing Value and water diffusion coefficient for PP-AAg-CmCi sample increased with the increasing of chitosan contained in the mixture of chitosan/collagen. The surface of the PP-AAg-CcCi sample was smooth; however, the Surface of the PP-AAg-CmCi sample was tough and cracky/loose. The higher water adsorption and water diffusion properties were Caused by the nature of agent for the PP-AAg-CmCi sample. The hydroxyl group contained on m-chitosan, which was prepared in this study, was higher than that contained on c-chitosan. The anti-bacterial properties of the PP-AAg-CmCi and PP-AAg-CcCi samples were all excellent. The products of the multi-layer material of PP-AAg-CmCi were expected to provide better services for wound dressing. (C) 2008 Elsevier B.V. All rights reserved