| 摘要: | 臺灣位於熱帶與亞熱帶地區，夏季常遭受梅雨、颱風或豪雨侵襲，引起淹水，造成花椰菜品質與產量受損。本研究以花椰菜耐熱品種’H41’、耐濕耐旱品種’H69’與耐寒品種’H71’為試驗材料，於’H41’移植後40天，’H69’、’H71’移植後65天之花球分生期，給予20℃與40℃之淹水與不淹水共4種處理0、6、12、24、48、72、96 hr，測定植株生長及葉片SPAD值、氣孔導度、葉綠素螢光Fv/Fm值、水分潛勢與離子滲漏率。高溫淹水48 hr期間，’H41’之Fv/Fm、水分潛勢與SPAD值皆表現出較佳耐受性。處理6與24 hr之植株葉片以全自動二維蛋白快速層析分離系統(ProteomeLabTM PF-2D)分析蛋白質。’H41’在20℃不淹水24 hr，20℃淹水處理6、24 hr，40℃不淹水6、24 hr與40℃淹水24 hr，分別有6、5、10、1、6、13個表現量有差異的相似蛋白質。’H69’於20℃不淹水、20℃淹水、40℃不淹水、40℃淹水24 hr，分別有10、3、15與15個表現量有差異的相似蛋白質。’H71’於20℃不淹水6 hr、20℃淹水6 hr、40℃淹水6 hr，分別有5、1與5個表現量有差異的相似蛋白質。這些蛋白質與細胞膜穩定性、胺基酸之生合成、光合作用、胺基酸轉譯、醣類代謝、訊息傳導有關。
Taiwan is located in the tropical and subtropical zones. Typhoons or heavy rain in summer season causes flooding, and damages the yield and quality of cauliflower (Brassica oleracea var. Botrytis L.).Cauliflower heat-resistant cultivar ‘H41’, flooding and drought-resistant cultivar ‘H69’, chiling-resistant cultivar ‘H71’ were used in the study. Forty days after transplanting of ‘H41’, 65 days after transplanting of ‘H69’ and ‘H71’, both flooding and non-flooding treatments at 20℃ and 40℃ were conducted. During treatment period, plant growth, SPAD, stomatal conductance, chlorophyll fluorescence Fv/Fm, water potential, and ion leakage percentage of leaves were measured at 0、6、12、24、48、72、96 hr. According to chlorophyll fluorescence, water potential, and SPAD values at 48-hour treatment, ‘H41’ plants showed better flooding and heat tolerance than others. The leaves of 6- and 24-hr treated plants were then used for proteomic analysis. For ‘H41’, there were 6, 5, 10, 1, 6, and 13 peaks with different protein expressions in leaves of non-flooding treatment at 24 hr and 20℃, flooding treatment at 6 and 24 hr and 20℃, non-flooding treatment at 6 and 24 hr and 40℃, and flooding 24 hr at 40℃, resrectively. For ‘H69’, there were 10, 3, 15, and 15 peaks with different protein expressions in leaves of non-flooding treatment at 20℃, flooding treatment at 20℃, non-flooding treatment at 40℃and flooding treatment at 40℃ for 24 hours, resrectively. For ‘H71’, there were 5, 1, and 5 peaks with different protein expressions in leaves of non-flooding treatment at 20℃, flooding treatment at 20℃, and flooding treatment at 40℃ and 24 hrs, resrectively. These proteins are related with cell membrane stability, amino acid biosynthesis, photosynthesis, amino acid translation, respiratory metabolism, and fatty acid synthesis. |
