文化大學機構典藏 CCUR:Item 987654321/20018
English  |  正體中文  |  简体中文  |  全文笔数/总笔数 : 46965/50831 (92%)
造访人次 : 12639708      在线人数 : 481
RC Version 6.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜寻范围 查询小技巧：
  • 您可在西文检索词汇前后加上"双引号"，以获取较精准的检索结果
  • 若欲以作者姓名搜寻，建议至进阶搜寻限定作者字段，可获得较完整数据
    •  进阶搜寻
    主页 ‧ 登入 ‧ 上传 ‧ 说明 ‧ 关于CCUR ‧ 管理 到手机版


    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/20018


    题名: 大量表現青花菜catalase基因以增強模式植物的耐熱性
    作者: 陳仕朋
    贡献者: 生物科技研究所
    关键词: 過氧化氫酶
    熱逆境
    活性氧族
    基因轉殖
    日期: 2010
    上传时间: 2011-10-31 12:39:54 (UTC+8)
    摘要: 植物曝露在各種不利的環境中會產生大量的活性氧族造成植物氧化傷害，其中在熱逆境中此情形更為嚴重。先前研究顯示，在熱逆境下相較於不耐熱的青花菜“優秀”品種，“欣樺1208”品種因為具有顯著的catalase (CAT1) 活性，而有較高的熱耐性，且其CAT1基因之cDNA全長為1768 bps，轉譯框架區域為1479 bps。而在本研究中，於熱逆境下，已從“欣樺1208”中再選殖出CAT2，為CAT1的異構體。
    本研究的目標是將CAT1以及CAT2在ubiquitin啟動子調控下，經由農桿菌LBA4404以及EHA105轉殖到目標植物中。阿拉伯芥轉殖株經過RT-qPCR分析後證明CAT確實在轉植株中具有大量表現。酵素活性分析結果顯示在熱逆境中AtCAT2-2轉殖株有最高的CAT活性，其CAT活性相對於未轉殖株顯著高出12.3倍，AtCAT2-2轉殖株經過西方墨點分析後也證實具有較高的蛋白質累積量。在熱逆境下，經過DAB染色證明轉殖株比未轉殖株呈現少量的過氧化氫累積。總之，阿拉伯芥中大量表現CAT基因能清除因熱逆境而過量產生的過氧化氫，並藉此增強其耐熱性。

    Oxidative stress is a major threat for plants exposed to various environmental stresses especially in heat stress. Previously, the CAT1 gene from heat-tolerant “Shin-Fa 1208” (SF1208) broccoli was cloned and up-regulated in response to heat-sensitive “Green Magic” broccoli under the heat stress conditions. Full-length of the broccoli CAT1 cDNA was 1768 bp, including 1479 bp of an open reading frame. In addition, CAT2 was a type of isoform to CAT1 from SF1208 under heat-induced environment, and was cloned in the current study.
    The objective of this study was to transfer the CAT1 and CAT2 cDNAs under the control of ubiquitin promoter to the host plants via Agrobacterium tumefaciens strain LBA4404 and EHA105. RT-qPCR analysis demonstrates that both BoCAT1 and BoCAT2 were over-expressed in the transgenic Arabidopsis. The CAT activity of AtCAT2-2 transgenic line was approximately 12.3-times higher than non-transgenic plant under the heat stress. The CAT protein of AtCAT2-2 was also shown highly accumulation in western blotting analysis. Compared with non-transgenic plants, lower level of heat-induced H2O2 accumulation was detected in leaves of transgenic plants with high level of CAT activity using DAB staining. These results indicate that overexpression of CAT gene in Arabidopsis could enhance the tolerance under heat stress by elimination of H2O2.
    显示于类别:[生物科技研究所 ] 博碩士論文

    文件中的档案:

    档案 描述 大小格式浏览次数
    index.html0KbHTML287检视/开启


    在CCUR中所有的数据项都受到原著作权保护.

    DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回馈