Polymerase Chain Reaction Identification of a Hymenopteran Insect in the Cornea: A Case Report

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Title:	Polymerase Chain Reaction Identification of a Hymenopteran Insect in the Cornea: A Case Report
Authors:	林憲忠葉文斌張丞賢傳耀賢程建中吳弘鈞王惠珠
Contributors:	生應系
Keywords:	角膜昆蟲異物聚合酶連鎖反應膜翅目
Date:	2006-03
Issue Date:	2011-01-27 11:53:37 (UTC+8)
Abstract:	昆蟲異物引起的角膜反應與該異物的成份有關。然而，之前報告過的角膜昆蟲異物很少以科學的方扶佐證。我們提出一位49歲男性，其左眼遭一未知之昆蟲撞擊。經緊急角膜切開術取出異物後，將檢體置於光學顯微鏡下觀察並作分子分析。將聚合酶連鎖反應(PCR)之產物作排序並比對，屬於膜翅目(Hymenoptera)昆蟲姬蜂總科的近綠物種。 The type of corneal injuries associated with insect encounters is related to the composition of the foreign body. However, previous reports on corneal foreign bodies as insects were rarely based on scientific evidence. Here, we report on a 49-year-old male who was stung in his left eye by an unknown insect. Emergent keratotomy was performed to remove the embedded corneal foreign body. The removed foreign body was observed under light microscopy, and a fragment of insect was suspected. The sample was sent for molecular analysis. The polymerase chain reaction product was sequenced, subjected to a BLAST search, and identified as an ichneumonoid member of the insect order Hymenoptera.
Relation:	The Kaohsiung Journal of Medical Sciences 22卷3期 P.143-148
Appears in Collections:	[Department of Applied Science of Living & Graduate Institute of Applied Science of Living ] journal articles

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