Effects of Antiglaucoma Drugs on Cellular Proliferation in Cultured Human Corneal Keratocytes

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Title:	Effects of Antiglaucoma Drugs on Cellular Proliferation in Cultured Human Corneal Keratocytes
Authors:	吳國揚王惠珠洪秀貞
Contributors:	生應系
Keywords:	抗青光眼藥細胞增生角膜纖維母細胞
Date:	2006-03
Issue Date:	2011-01-27 11:51:34 (UTC+8)
Abstract:	本實驗主要是研究不同的抗青光眼藥包括timolol，betaxolol，carteolol，levobunolol，dipivefrin and pilocarpine對人類角膜纖維母細胞生長的影響。人類角膜纖維母細胞經RPMI-1640及10%胎牛血清培養後加入經1/10，1/100及1/1,000倍稀釋的抗青光眼藥，經過100分鐘培養後測試細胞對[3H]thymidine的攝取量。結果發現細胞的生長只被1/10倍稀釋的timolol，betaxolol，carteolol，levobunolol，dipivefrin及pilocarpine抑制到只有控制組的63%，18%，87%，68%，55%及67%。而且0.01 mg/mL的benzalkonium chloride防腐劑也會明顯抑制人類角膜纖維母細胞的生長。因此本實驗顯示高濃度的抗青光眼藥可能因為含benzalkonium chloride防腐劑而會抑制人類角膜纖維母細胞的增生。 The purpose of this study was to investigate the effects of various antiglaucoma drugs, including timolol, betaxolol, carteolol, levobunolol, dipivefrin, and pilocarpine, on cellular proliferation in cultured human corneal keratocytes. Human corneal keratocytes were cultured with RPMI-1640 medium containing 10% fetal bovine serum. Antiglaucoma drugs were prepared from original concentrations to dilutions of 1/10, 1/100, and 1/1,000. After exposure to drugs for 100 minutes, cellular proliferation was estimated by [3H] thymidine uptake methodology. It was found that cellular proliferation in corneal keratocytes was inhibited by only a 1/10 dilution of various drugs including timolol, betaxolol, carteolol, levobunolol, dipivefrin, and pilocarpine. The [3H]thymidine uptake values were significantly inhibited to 63%, 18%, 87%, 68%, 55%, and 67% by a 1/10 dilution of the above drugs. However, the cellular proliferation was also significantly suppressed by 0.01 mg/mL of benzalkonium chloride preservative. It is shown that the inhibition of cellular proliferation by high concentrations of antiglaucoma drugs may result from the benzalkonium chloride preservative contained in these drugs.
Relation:	The Kaohsiung Journal of Medical Sciences 22卷3期 P.120-125
Appears in Collections:	[Department of Applied Science of Living & Graduate Institute of Applied Science of Living ] journal articles

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