Cellular Cytotoxicity of Antiglaucoma Drugs in Cultured Corneal Endothelial Cells

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Title:	Cellular Cytotoxicity of Antiglaucoma Drugs in Cultured Corneal Endothelial Cells
Authors:	吳國揚王惠珠洪秀貞
Contributors:	生應系
Keywords:	抗青光眼藥細胞毒性角膜內皮細胞
Date:	2007-03
Issue Date:	2011-01-27 11:41:50 (UTC+8)
Abstract:	本實驗主要是研究不同的抗青光眼藥包括betaxolol、timolol、levobunolol、carteolol、brimonidine、dipivefrin、dorzolamide、brinzolamide、latanoprost、unoprostone及pilocarpine對體外培養牛角膜內皮細胞毒性的影響。細胞以1/100、1/1,000及1/10,000稀釋的上述眼藥水培養100分鐘後，以lactate dehydrogenase (LDH)的釋出量測試不同的眼藥水對細胞的毒性作用。結果發現細胞經過1/100稀釋的betaxolol、brimonidine、dorzolamide、dipivefrin、latanoprost及unoprostone培養100分鐘後會明顯增加細胞釋放lactate dehydrogenase的量到控制組的130%、123%、145%、157%、128%及237%，而且細胞經0.001、0.0001及0.00001mg/mL防腐劑benzalkonium chloride培養100分鐘後並不會改變角膜內皮細胞釋放lactate dehydrogenase的量。因此本實驗顯示高濃度的抗青光眼藥水可能會對角膜內皮細胞產生毒性作用。 In this study, the various antiglaucoma drugs including betaxolol, timolol, levobunolol, carteolol, brimonidine, dipivefrin, dorzolamide, brinzolamide, latanoprost, unoprostone, and pilocarpine were used to investigate the effects of cellular cytotoxicity in cultured bovine corneal endothelial cells. After exposure to the drugs in three dilutions, 1/100, 1/1,000, and 1/10,000, for 100 minutes, cells were estimated based on the release assay of lactate dehydrogenase (LDH) enzyme. It was found that cellular LDH was significantly released in the medium only at 1/100(superscript th) dilution of betaxolol, brimonidine, dorzolamide, dipivefrin, latanoprost and unoprostone to l30%, 123%, 145%, 157%, 128% and 237%, respectively, compared with controls upon exposure to drugs for 100 minutes. Moreover, benzalkonium chloride preservative at the concentrations ranging from 0.001 to 0.00001mg/mL did not affect cellular LDH release in bovine corneal endothelial cells. These results indicate that high concentrations of antiglaucoma drugs may induce cytotoxicity in corneal endothelial cells.
Relation:	The Kaohsiung Journal of Medical Sciences 23卷3期 P.105-111
Appears in Collections:	[Department of Applied Science of Living & Graduate Institute of Applied Science of Living ] journal articles

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