研究內皮素－1(endothelin-1、ET1)在接受過雷射角膜切除術(Photorefractive keratectomy、PRK)之後的兔子角膜傷口癒合的影響。一共有十八隻紐西蘭白兔接受PRK，其右眼點以ET1而左眼點以磷酸緩衝鹽溶液(phosphate-buffered salt solution、PBS)。我們紀錄角膜上皮傷口大小、角膜混濁、與角膜厚度。我們利用組識免疫化學法(immunohistochemistry)來研究角膜的細胞外基質(extracellular matrixes)包括第三、四、七型膠原蛋白(collagen)、軟骨素(chondroitin sulfate)、與纖維連結蛋白(fibronectin)。在雷射過後十二週與十四週，在點以ET1的眼睛仍然可以發現有纖維連結蛋白，但是對照組的則沒有。ET1會加速兔子角膜上皮傷口的癒合。點以ET1的眼睛與對照組在角膜混濁、角膜厚度、與其他的細胞外基質則沒有明顯的差異。ET1可以增加纖維連結蛋白在角膜基質的堆積而且可以促進角膜上皮傷口的癒合。ET1促使纖維連結蛋白在角膜的增加也許可以解釋其增進上皮傷口癒合的可能機制。
The objective was to study the effects of endothelin-1 (ET1) on corneal wound healing after photorefractive keratectomy (PRK) in rabbit corneas. Following PRK, 18 New Zealand white rabbits were treated with ET1 in the right eyes and with phosphate-buffered salt solution (PBS) in the left eyes. Corneal epithelial wound size, corneal haze and corneal thickness were recorded. Corneal extracellular matrixes, including collagen types 3, 4 and 7, chondroitin sulfate and fibronectin, were investigated using immunohistochemistry study. ET1 increased the rate of healing of corneal epithelial wounds in rabbits. Anti-fibronectin fluorescence was present at week 12 and week 24 in ET1-treated eyes but not in the control eyes. There were no significant differences in corneal haze, corneal thickness and changes in other extracellular matrixes between ET1-and PBS-treated eyes. ET1 can enhance the deposition of fibronectin in corneal stroma and promote corneal epithelial wound healing after PRK. The increase in fibronectin probably explains the increased healing rate of corneal epithelial wounds.
關聯:
The Kaohsiung Journal of Medical Sciences 24卷5期 P.254-261
