三個含有水稻醇溶蛋白RP3/GUS chimeric gene inserts 之轉植菸草,植株(品系RP3/2,RP3/7及RP3/18)已被分離及建立懸浮細胞培養(Yang et al.,1999)，細胞品系間差異亦被觀察到(Chen et al.,1999)。在這彷報告中我們使用色素如類葫蘿蔔素、葉綠素a及b作為標誌探討RP3起動子活性與細胞色素含量的可能關聯，并且發現此二者於細胞品系間有正相關性。RP3/2的懸浮細胞具有最多的色素含量及最大的GUS表現量，RP3起動子活性在此懸浮細胞內12天的繼代培養過程中逐漸的減少，而且與細胞色素含量下降量平行。於RP3/18，於細胞生長過程該二者亦顯示平行的關聯性且保持相當的持平。由這些實驗結果顯示水稻醇溶蛋白RP3起動子活性在轉殖菸草懸浮細胞品系間及品系內與色素含量有正相關性。我們對該關聯之意義及可能的解釋提出一些看法。
Three transgenic tobacco regenerants (lines RP3/2, RP3/7 and RP3118) harboring rice RP3IGUS chimeric gene inserts in their genomes were isolated previously and used to establish cell suspension cultures (Yang et al., 2000). A differential GUS expression pattern together with morphological, biochemical, and molecular variations were observed among these cell lines (Chen et al., 1999). In this report we used pigments such as carotenoids and chlorophylls a and b as markers to study the possible relationship between RP3 promoter activity and cellular pigment contents, and a positive association was found among these cell lines. RP312, which contained the highest cellular pigment contents, also exhibited the highest GUS expression level. The RP3 promoter activity in RP3/2 continuously decreased and was parallel with the reduction of pigment contents within a 12-day growth period after subculture. In RP3/18, both also showed a parallel association and remained relatively constant during cell growth. Based on these results, a positive association between RP3 promoter activity and cellular pigment content was found among and within cell lines. The meaning of the association and its possible explanation are discussed.
關聯:
Botanical Bulletin of Academia Sinica 41卷2期 P.93-98
