Surface plasmon resonance-based immunosensor with oriented immobilized antibody fragment on a mixed self-assembled monolayer for the determination of staphylococcal enterotoxin B.

CCUR > College of Environmental Planning & Bioresources > College of Agriculture > Graduate Institute of Biotechnology > journal articles > Item 987654321/18698

Please use this identifier to cite or link to this item: https://irlib.pccu.edu.tw/handle/987654321/18698

Title:	Surface plasmon resonance-based immunosensor with oriented immobilized antibody fragment on a mixed self-assembled monolayer for the determination of staphylococcal enterotoxin B.
Authors:	蔡文琦白璧如
Contributors:	生科所
Keywords:	Surface plasmon resonance Mixed self-assembled monolayers Antibody fragments Staphylococcal enterotoxin B Immunosensor
Date:	2009-07
Issue Date:	2011-01-17 14:21:18 (UTC+8)
Abstract:	An immunosensor based on surface plasmon resonance (SPR) with a mixed self-assembled monolayer (SAM) was developed to determine staphylococcal enterotoxin B (SEB). The SAM on a gold surface was fabricated by adsorbing a mixture of 16-mercapto-1-hexadecanoic acid (16-MHA) and hexanethiol at various molar ratios. Initially, full-length anti-SEB was randomly immobilized onto the SAM to form the immunosensing surface. Through optimization of surface functionalization and anti-SEB immobilization, the SPR sensors can be applied to the determination of SEB in a linear range of 0.01 ~ 1.0 μg.mL−1. Furthermore, a smaller antibody fragment (F(ab)’) was generated and immobilized randomly (via amino groups) or in an oriented manner (via −SH groups) to form the immunosensing surface. The oriented immobilization of F(ab)’ led to a 50% increase in the antigen binding efficiency compared to randomly immobilized covalent F(ab’) fragments. The resulting calibration curve showed higher sensitivity. In addition, the specificity and applicability of the proposed immunosensor to milk samples were also demonstrated. Furthermore, the sensor can be regenerated using 0.1 M HCl, and 70% of the initial response was maintained over 3 cycles.
Relation:	Microchimica Acta Volume 166, Numbers 1-2 P.115-122
Appears in Collections:	[Graduate Institute of Biotechnology ] journal articles

Files in This Item:

File	Description	Size	Format
index.html		0Kb	HTML	537	View/Open

Loading...