SPR-based immunosensor for determining staphylococcal enterotoxin A

文化大學機構典藏 CCUR > 環境規劃暨生物資源學院 > 農學院 > 生物科技研究所 > 期刊論文 > Item 987654321/18697

jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/18697

题名:	SPR-based immunosensor for determining staphylococcal enterotoxin A
作者:	蔡文琦賴怡潔
贡献者:	生科所
关键词:	Surface plasmon resonance Mixed self-assembled monolayers Staphylococcal enterotoxin A Immunosensor
日期:	2009-02
上传时间:	2011-01-17 14:13:21 (UTC+8)
摘要:	An immunosensor for determining staphylococcal enterotoxin A (SEA) was developed on the basis of surface plasmon resonance (SPR). The sensor surfaces were constructed from various thiol mixtures of different molar ratios of 16-mercapto-1-hexanol (16-MHA) to 6-mercapto-1-hexanol (6-MCH). The surface functionalized with a 1:20 mixed self-assembled monolayer (SAM) exhibited the best result in terms of SEA detection sensitivity. Through optimization of anti-SEA immobilization, the SPR sensor could detect SEA in phosphate-buffered saline buffer in a linear range from 100 to 1000 ng/ml. Furthermore, studies of recovery and matrix effects were performed to evaluate the suitability of the developed immunosensor for directly analyzing SEA in milk samples. Results indicated that the mixed SAM-based SPR immunosensor has the potential for the specific detection of SEA.
關聯:	Sensors and Actuators B Volume 136, Issue 1 P.8-12
显示于类别:	[生物科技研究所 ] 期刊論文

文件中的档案:

档案	描述	大小	格式	浏览次数
index.html		0Kb	HTML	661	检视/开启

在CCUR中所有的数据项都受到原著作权保护.