RAPD Variation in Relation to Population Differentiation of Chamaecyparis formosensis and Chamaecyparis taiwanensis

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Title:	RAPD Variation in Relation to Population Differentiation of Chamaecyparis formosensis and Chamaecyparis taiwanensis
Authors:	黃士穎林惠文郭怡秀林讚標
Contributors:	生科所
Keywords:	紅檜台灣扁柏族群分化逢機擴增多型性DNA
Date:	2001-07
Issue Date:	2011-01-17 09:02:08 (UTC+8)
Abstract:	本文探討紅檜與台灣扁柏之族群分化問題。紅檜與台灣扁柏的樓蘭山與阿裏山兩個族群以逢機擴增多型性DNA分子標記研究族群分化之問題。RAPD變異以Shannon′s phenotypic index來估算Ho。無論是紅檜或台灣扁柏之樓蘭山族群都較阿裏山族有較高之遺傳變異。上述結果可能與樓蘭山爲紅檜與台灣扁柏之大族群有蘭。基於RAPD之分析發現紅檜樓蘭山與阿裏山族群間有15.14%之分化值，而台灣扁柏樓蘭山與阿裏山族群間分化值爲14.73%。RAPD基因座之高的遺傳變異與族群分化程度可能代表紅檜與台灣扁柏之動態演化有關。 The population differentiation of Chamaecyparis formosensis and Chamaecyparis taiwanensis based on random amplified polymorphic DNA (RAPD) variation is described. Two populations (Chilanshan and Alishan) of these two Chamaecyparis species are investigated for population differentiation. Shannon's phenotypic index was used to estimate Ho of these two Chamaecyparis species based on RAPD variation. RAPD analysis showed that the Chilanshan population had higher genetic diversity than the Alishan population in both Chamaecyparis species. These results correlated with the large population found in Chilanshan for both species. Based on RAPD analysis, there was 15.13% population differentiation between Chilanshan and Alishan of C. forinosensis compared with 14.73% for C. taiwanensis. Higher levels of genetic variation and population differentiation indicated dynamic evolution in these two Chamaecyparis species in Taiwan as revealed by variation at RAPD loci.
Relation:	Botanical Bulletin of Academia Sinica 42卷3期 P.173-179
Appears in Collections:	[生物科技研究所 ] 期刊論文

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