文化大學機構典藏 CCUR:Item 987654321/17648
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    jsp.display-item.identifier=請使用永久網址來引用或連結此文件: https://irlib.pccu.edu.tw/handle/987654321/17648


    题名: 台灣肖楠之組織培養
    其它题名: Tissue Culture of Calocedrus formosana (Florin) Florin
    作者: 向韻如
    吳宗賢
    黃士穎
    林敏宜
    贡献者: 森保系
    关键词: 台灣肖楠
    癒合組織培養
    微體繁殖
    胚培養
    日期: 2004-12
    上传时间: 2010-11-18 11:04:59 (UTC+8)
    摘要: 本試驗以台灣肖楠之癒合組織、成熟胚、成熟胚及胚乳、未成熟胚、新鮮毬果等為培殖體,嘗試誘導其芽體及胚發生。在含0.05mg/L NAA與1mg/L BA之WPM基礎培養基中,芽體誘導之效果較好;添加0.05mg/L 2,4-D與2mg/L BA則能夠誘導出較多之體胚;而2,4-D較NAA容易誘導器官分化,配合添加BA濃度愈高愈能使已褐化之癒合組織長出白色鬆軟之癒合組織,且在低濃度時,可誘導出芽體或體胚之分化。在胚之表面消毒試驗,未成熟胚可達零污染。以椰子乳添加於WPM基礎培養基中進行胚培養4星期,並沒有任何生長或分化之情形發生。台灣肖楠毬果經培養8星期後,會分泌大量二次代謝物,且培殖體無任何反應。
    Callus, mature embryos, immature embryos and fresh corns of Calocedrus formosana were used as explants. Multi-buds were induced from callus cultured in WPM medium supplemented with 0.05mg/L NAA and 1mg/L BA. Somatic embryos were induced in WPM medium supplemented with 0.05mg/L 2,4-D and 2mg/L BA. Callus was easier to induce organogenesis while cultured in WPM medium supplemented with 2,4-D than NAA. The higher concentration of 2,4-D with lower concentration of BA can induce soft and white callus from callus has browning. In the surface sterilization, immature embryo were decontaminated completely, the mature embryo removes the endosperm were obtained the lower pollution rate. After 8 weeks, culture the vigor of fresh corns dissolved gradually. There was no response in the embryo cultured in WPM medium supplemented with coconut milk.
    關聯: 華岡農科學報 14期 P.17-31
    显示于类别:[農學院] 學報-華岡農科學報

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