本研究製備過氧化鈣/靛氰綠/海藻膠/氧化鐵奈米複合材料，目的是為了改善腫瘤缺氧微環境導致降低光動力治療效率的影響。首先，利用共沉澱法製備氧化鐵奈米顆粒，接著將粒子表面以共價鍵修飾上海藻膠，再將光敏劑(靛氰綠)包覆至海藻膠/氧化鐵奈米粒子內部，並加入鈣離子與表面海藻膠螯合，最後加入過氧化氫與材料表面鈣離子作用，形成可產生氧氣的過氧化鈣/靛氰綠/海藻膠/氧化鐵奈米複合材料。並利用穿隧式電子顯微鏡、紫外光-可見光光譜儀、螢光光譜儀、粒徑及表面電位分析儀驗證其物理及化學性質。此奈米複合材料在經過808 nm連續式雷射照射後可藉由材料本身釋放的氧氣及內部光敏劑來產生更多單態氧，並且在體外細胞研究中也顯示出，此奈米複合材料確實可以有效增益光動力治療之效能。
In this study, calcium peroxide/indocyanine green/alginate/iron oxide nanocomposites were prepared to improve the lower efficiency of photodynamic therapy caused by the hypoxic microenvironment within the tumor. First, iron oxide nanoparticles were prepared by co-precipitation method and then modified with alginate on the particle surface by a covalent bond. Then, indocyanine green (ICG) as the photosensitizer was encapsulated into the alginate/iron oxide nanoparticles and calcium ions were used to inhibit ICG leaching by formation of cross-linking network. Finally, hydrogen peroxide was added to react with the calcium ions on the surface of the alginate/indocyanine green/iron oxide nanoparticles to form the calcium peroxide/indocyanine green/alginate/iron oxide nanocomposite that can generate oxygen. TEM, UV-vis, DLS, EDS, fluorescent microscopy, zeta-potential were used to characterize the physical and chemical properties of nanocomposites. These nanocomposites can certainly generate additional oxygen from CaO2 and then produce more singlet oxygen from ICG after 808 nm laser irradiation. The enhanced photodynamic therapy of nanocomposites was also evaluated by in-vitro (cell) tests.
