葉綠素螢光可作為偵測光合作用胞器之激發能量，並提供光合作用機制及調節的觀察。但目前缺乏利用葉綠素螢光模組偵測水稻植株在田間環境之快速及非破壞性量測資料。本研究比較水稻葉片之葉綠素螢光及二氧化碳吸收，包括比較兩者之不同參數、日變化及提高二氧化碳濃度之影響，結果顯示葉片之淨同化速率可由螢光參數之電子傳遞速率作精確估算。基於此理由，相較於一般利用二氧化碳消耗測定光合作用速率，本研究證實監測葉綠素螢光更可作為篩選預測作物潛在生產力及逆境耐性之有用指標。
Chlorophyll fluorescence has been used to probe the fate of excitation energy within the photosynthetic apparatus and provide insight into the mechanism and regulation of photosynthesis in vivo. However, no rapid, non-invasive measurement data of chlorophyll fluorescence from the field environment on rice plants was available. In this study, was investigated the change of chlorophyll fluorescence and gas exchange of rice leaves in the field, including the relationship between in various parameters, diurnal change in field and influenced by elevated CO2 concentration. The results showed the rate of net assimilation of CO2 of rice leaves can be accurately estimated from the measurements of electron transport rate (ETR) using chlorophyll fluorescence techniques. As compared to common methods measuring the photosynthesis rate based on CO2 consumption, the approach monitoring chlorophyll fluorescence verified in this study is a useful tool to screen crops with potential productivity and stress tolerance.
