Today, news reading is increasingly essential in terms of staying up-to-date and more deeply embedded in people’s daily lives than it has ever been. The aim of the study is to investigate how different types of medium influence on readers' news reading ability through Thai College students as the study sample. The experimental study is conducted with online news, print newspapers, and control groups. Participants will read an article then fill in the questionnaire provided online. The questionnaire contains questions of demographic and news reading ability that includes reading comprehension vocabulary and text test. The total study sample is 350 respondents. The statistics for the study described by T-Test and one way ANOVA with Post HOC for the 3 groups of medium will be employed to test hypotheses after the test results are adopted from SPSS. The results show that there is a significant difference on readers' news reading ability through different types of medium. The print newspaper readers have higher reading ability than online news readers and control group even though the scores from reading comprehension test including vocabulary test and text comprehension test present online news readers beat up print newspaper readers when fewer students failed.